Automated cell counter
& cell viability analyzer
NORMA 4S
High throughput at µl scale designed for cell count of small scale parallel bioreactors
For research use only (RUO). Not for use in diagnostic procedures.
SAMPLE PREPARATION FREE
SHORT TIME RESULTS
HIGH REPEATABILITY
LOW SAMPLE VOLUME
MATCH WITH REFERENCE TRYPAN BLUE METHOD
100% AUTOMATIC SOLUTIONS ON YOUR PARALLEL MICRO BIOREACTORS
The NORMA 4S, based on the new lens-less technology, is a fully automatic cell counter for high throughput cell culture monitoring. It rapidly measures cell concentration and viability without repeatability compromise. The NORMA 4S provides an unmatched accuracy and precision thanks to its wide fields of view (29.4 mm2) that allows counting several thousands of cells within a single sample image! No need to adjust focus, brightness or any other parameters, our algorithm will reconstruct cells wherever cells are located… from a few micrometres to some millimetres! There is no sample preparation: no dilution and no Trypan Blue! You just have to take about 3µL of your sample and to deposit it on the measuring slide. The NORMA 4S is ready for receiving the sample from the robotic arms of a parallel micro bioreactor, an automatic sampler or even a pipette.
Features of the NORMA 4s
- Automatic cell count and viability
- Sample preparation free
- Short time results
- High repeatability
- Low sample volume
- Match with reference Trypan Blue method
Automatic cell count
Viability
Cell size distribution
Ratio aspects
Growth curves
Ready for seamless integration in high-throughput automated micro bioreactors
The NORMA 4S is composed of a refillable cartridge of 36 4-chambers slides allowing 144 samples to be made. A slide from the cartridge is automatically put on the sample area where the sample can be drop and start the measurement. When it’s done, the slide is thrown in the trash and a new one is ready to receive four another samples. The technology directly acquires the light diffraction properties of each individual cells through their hologram images without any objectives, lens of focus settings. Living and dead cells have significant holographic patterns that can be distinguished and accurately counted. The measurements are analysed by the HORUS software.
The NORMA 4S works with precise calibrated measurement chambers (1) constructed on single-use slides (2). The refillable slide cartridge (3) is ready to run 144 samples (4) without user interaction.
The integration of the NORMA 4s into the Ambr15
The NORMA 4S is fully adapted to the Sartorius Ambr15 automated micro bioreactor.
HORUS Software Included
Our best software for cell counting and cell analysis !
HORUS Software is included with the acquisition of our products and is regularly updated by our development team.
TECHNICAL SPECIFICATIONS
| Cells | Mammalian cells: CHO - JURKAT - HEK293 - YT - NIH3T3 - PC12 - HELA - VERO |
| Insect cells: SF9 - HIGHFIVE | |
| Concentration range | 10⁴-4.10⁷ cell/ml |
| Cell size range | 7 - 50 μm |
| Sample volume | 3 - 13 μl |
| Numbers of sample | 4 samples/slide - 144 samples/box |
| Viability determination | Light diffraction |
| Counting time | 15 seconds |
| Image | .PNG / .BMP / .TIFF / .RAW / .AVI |
| Dimensions | 30 x 11,5 x 25 cm |
| Enclosure | Stainless steel, POM |
| Weight | 4 kg |
| Power supply | USB + 24 V DC (110 - 240 V AC) |
| Pharmaceutical industries | 21 CFR part 11 & IQ/OQ |
| Integration | Ambr15® |
ACCESSORIES AND CONSUMABLES
| HORUS Software |
| Laptop computer |
| NORMA 4S Cell counting slides – 4 chambers: 20 µm (from 2.1 x10^6 to 5.1x10^7 cells/ml) |
| NORMA 4S Cell counting slides – 4 chambers: 100 µm (from 10^4 to 5.1x10^6 cells/ml) |
F.A.Q.
The NORMA range allows you to count your cells and determine their viability and diameter. You will get automatically saved images.
No, only your raw sample is needed in order to do the cell count and viability analysis.
Yes, all our products are driven directly by our HORUS software. The data is also analysed by it.
Yes, all our products are 21 CFR.
By default, the image format is JPEG. It is possible to have images in PNG, BITMAP, TIFF. The results can be exported in CSV, XML, XLSX format.