LIVE CELL IMAGING INSIDE THE INCUBATOR

CYTONOTE 6W

TIME-LAPSE IMAGES OF CELLS AND REAL TIME ANALYSIS FROM INSIDE YOUR INCUBATOR

Specifically designed for easy parallel monitoring of 6 well plate cultures

For research use only (RUO). Not for use in diagnostic procedures.

LABEL FREE & HIGH CONTRAST

ALWAYS IN FOCUS

SETTINGS FREE

HUGE FIELD OF VIEW

CELL CONFLUENCE DETERMINATION

CELL BASED ASSAY, PROLIFERATION, MIGRATION, TRACKING, WOUND HEALING...

THE SIMPLEST LIVE CELL-IMAGING SYSTEM DESIGNED FOR THE MONITORING UP TO 6 CELL CULTURES IN PARALLEL

The CYTONOTE 6W uses our patented lens-less imaging technology in a tiny device design to fit within any incubator. The CYTONOTE product range simplifies live cell-imaging technique and transforms the complex and expensive microscope into a cost-effective solution.

The CYTONOTE 6W is the simplest live cell-imaging system designed for recording cell movies and analyzing a variety of cell culture from inside the incubator. The innovative and patented “lens-less imaging” technology pushes the boundaries of microscopy with its super wide field of view and its capability to capture and analyze precisely several thousands of cells without any focus and brightness settings.

FEATURES CYTONOTE 6W

In direct from your incubator
Time-Lapses & Movies
High contrast
Always in focus
Settings free
Huge field of view
Robust
Many cell culture application

CYTONOTE 6W APPLICATIONS

Real-Time Imaging of your cell culture
Up to 6 cell cultures in parallel
Time-Lapses and Movies of your cells
Confluence analysis
Growth curves
Migration test
Proliferation test
Cell morphology
Cell population
Cell attachment / detachment
Cell tracking

HOW TO USE OUR CELL IMAGER ANALYSER?

The CYTONOTE 6W has been designed to be compatible with all Petri dishes, T-Flask and slides. Select your container and place it on the CYTONOTE 6W. The default acquisition time between two measurements is 20 minutes. However, this time can be changed at your convenience and thus you can adapt the time periods for the realization of time-lapses of your cells.

As explained above, no adjustment of parameters such as focus or brightness is necessary. Our algorithm will reconstruct the cells wherever they are located in the container. These features make the CYTONOTE 6W robust to temperature variations, cell drop inside the Matrigel, or any other parameters that may affect the traditional focus adjustment.

WHAT ARE THE RESULTS ?

The CYTONOTE 6W works on an ultra-wide field of view where all data recovered from picture represent your cell culture. The image analysis and results from the CYTONOTE 6W are performed by the HORUS dedicated Software. HORUS is application oriented: it provides quantitative confluence determination, cell size or cell tracking. Full field image (30mm²) of the samples are stored and can be accessed and zoomed at any time.

HORUS Software Included

Our best software for cell counting and cell analysis !

HORUS Software is included with the acquisition of our products and is regularly updated by our development team.

TECHNICAL SPECIFICATIONS

Cells	Eucaryotic cells : Adherent monolayer, Suspension cell at bottom of culture ware or in micro-slides, 3D spheroids
Media	Liquid or Semi-solid (collagen)
Culture Vessels	Standard plastic petri dish, culture flask, multiwell plate, max height 55mm
Resolution	1 micron
Field of view	30 mm² (6,5 mm x 6,5 mm)
Working distance	0 to 5 mm
Image rate	1,5 image/min
Light source	LED
Sensor	CMOS 10 Mpix
Image	.JPEG / .PNG / .BITMAP / .TIFF
Enclosure	Stainless Steel
Dimensions	17 x 13 x 10,5 cm
Weight	2 kg
Power Supply	USB
Pharmaceutical industries	21 CFR part 11 & IQ/OQ

ACCESSORIES AND CONSUMABLES

HORUS Software
21 CFR part 11
Scratch Assay analysis module for HORUS Software
Cell Tracking analysis module for HORUS Software
Cell Tube Formation analysis module for HORUS Software
Cytonote cable – 2 meters
Laptop computer
IQ/OQ

TESTIMONY

“What I like most about the CYTONOTE is the tracking of thousands of cells. With the microscope, you can only follow a few hundred cells… If I could not track so many cells, I would never have been able to see this new cell rhythm. This allows the discovery of new phenomena that cannot be seen with other microscopes, and this without labelling or preparation and in direct from the incubator. The 6-well system is excellent for parallel culture monitoring, especially when testing with drugs! It’s very convenient! It’s a tool that is now part of our protocols! It’s essential to have several instruments to obtain all types of information. The CYTONOTE allows to bring a lot of additional information that is not possible to get with other microscopes.”

Lamya GHENIM

CEA - 2021

FAQ

What are the advantages of holography compared to a microscope?

By this product technology, there is a wide field of view, no manual adjustment step and the results are obtained in seconds.

Do you need a PC to operate your instruments?

Yes, all our products are driven directly by our HORUS software. The data is also analysed by it.

Is the software compatible with 21 CFR?

Yes, all our products are 21 CFR.

What type of image export is possible?

By default, the image format is JPEG. It is possible to have images in PNG, BITMAP, TIFF. The results can be exported in CSV, XML, XLSX format