AUTOMATIC CELL COUNTER AND VIABILITY ANALYZER
High throughput at µl scale designed for cell count of small scale parallel bioreactors
MATCH WITH REFERENCE
TRYPAN BLUE METHOD
PRODUCT OVERVIEW – NORMA 4S
100% AUTOMATIC SOLUTIONS ON YOUR PARALLEL MICRO BIOREACTORS
The NORMA 4S, based on the new lens-less technology, is a fully automatic cell counter for high throughput cell culture monitoring. It rapidly measures cell concentration and viability without repeatability compromise. The NORMA 4S provides an unmatched accuracy and precision thanks to its wide fields of view (29.4 mm2) that allows counting several thousands of cells within a single sample image! No need to adjust focus, brightness or any other parameters, our algorithm will reconstruct cells wherever cells are located… from a few micrometres to some millimetres! There is no sample preparation: no dilution and no Trypan Blue! You just have to take about 3µL of your sample and to deposit it on the measuring slide. The NORMA 4S is ready for receiving the sample from the robotic arms of a parallel micro bioreactor, an automatic sampler or even a pipette.
FEATURES OF THE NORMA 4S
- Automatic cell count and viability
- Small device
- Sample preparation free
- Short time results
- High repeatability
- Low sample volume
- Match with reference Trypan Blue method
READY FOR SEAMLESS INTEGRATION IN HIGH-THROUGHPUT AUTOMATED MICRO BIOREACTORS
The NORMA 4S is composed of a refillable cartridge of 36 4-chambers slides allowing 144 samples to be made. A slide from the cartridge is automatically put on the sample area where the sample can be drop and start the measurement. When it’s done, the slide is thrown in the trash and a new one is ready to receive four another samples. The technology directly acquires the light diffraction properties of each individual cells through their hologram images without any objectives, lens of focus settings. Living and dead cells have significant holographic patterns that can be distinguished and accurately counted. The measurements are analysed by the HORUS software.
The NORMA 4S works with precise calibrated measurement chambers (1) constructed on single-use slides (2). The refillable slide cartridge (3) is ready to run 144 samples (4) without user interaction.
THE INTEGRATION OF THE NORMA 4S INTO THE AMBR15
The NORMA 4S is fully adapted to the Sartorius Ambr15 automated micro bioreactor.
For more details, go to the “Applications” Tab!
TECHNICAL SPECIFICATIONS – NORMA 4S
|Concentration Range||10^4 – 4.10^7 cell/ml|
|Sample Volume||3-13 µl|
|Cell Size Range||7-50 μm|
|Viability Determination||Light Diffraction|
|Counting Time||15 seconds|
|Enclosure||Stainless steel, POM|
|Dimensions||30 x 11.5 x 25 cm|
|Power Supply||24 V DC (110-240 V AC power converter included)|
ACCESSORIES AND CONSUMABLES – NORMA 4S
|Cell counting slide 20µm – 4 Chambers (from 2x10e6cells/mL to 5x10e7cells/mL)||Laptop Computer|
|Cell counting slide 100µm – 4 Chambers (from 10e4cells/mL to 5x10e6cells/mL)||Remote control TCP/IC module for HORUS|
|Beads Control 1M1||IQ/OQ|
|Beads Control 1M10||21 CFR Part 11|
|Beads Control 8M1|
|Beads Control 8M10|
APPLICATIONS – NORMA 4S
MEDIA – NORMA 4S