Viable cell density monitoring in bioreactor
Material and method
Figure 1 : The NORMA XS versus the Vi-Cell XR Evaluation on perfusion and fed-batch processes (data courtesy of GSK)
A strong correlation between the NORMA XS and the Beckman Coulter Vi-Cell XR on the perfusion and fed-batch processes
Acknowledgement : These results are part of a project that has received funding from the Innovative Medicines Initiative 2 Innovative Medicines Initiative 2 Joint Undertaking (grant agreement No 777397 for funding. This Joint Undertaking receives the support from the European Union’s Horizon 2020 research and innovation programme and EFPIA partners Bayer, Byondis , GSK, Pfizer, Rentschler Biopharma, Sanofi and UCB.
Disclaimer : This communication reflects the author’s view and neither IMI nor the European Union, EFPIA, or any Associated Partners are responsible for any use that may be made of the information contained therein.
The Viable Cell Density and cell viability correlation between the Nova BioProfile FLEX, Beckman Coulter Vi-Cell XR and NORMA XS on 12 parallel fed-batch bioreactors
Figure 2 : Viable cell density (A) and cell viability (B) correlation between the lensless imaging technique NORMA XS and the trypan blue reference instrument Beckman Coulter Vi-Cell, for N=84
Figure 3 : Viable cell density (A) and cell viability (B) correlation between the lensless imaging technique NORMA XS and the trypan blue reference instrument Nova BioProfile FLEX, for N=84
High reproducibility of the NORMA XS
Single sample injected in 12 different chambers and measured with 1 NORMA XS
Single sample injected in 1 chamber and measured with 5 separate NORMA XS
The project leading to this application has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under Grant Agreement n°777397. This Joint Undertaking receives the support from the European Union’s Horizon 2020 research and innovation programme and EFPIA.
(1) C. Allier, T. Bordy, et al., “Label-free cell viability assay using lens-free microscopy”
(2) G. Esteban, M. Pisaneschi, J. Cubeta and D. Sergeant, “Viable cell density monitoring in bioreactor with lensless imaging”, ESACT conference 2017 poster
(3) G. Esteban, M. Hill, « Viable Cell Density and Viability in High-Throughput Cell Culture Applications”, Cambridge Healthtech Institute Webinar 2020