TIME-LAPSE IMAGES OF CELLS AND REAL TIME ANALYSIS FROM INSIDE YOUR INCUBATOR
CYTONOTE 1W
A small footprint device that stays always in focus with Petri dishes, T-flasks, slides or microfluidic chips
CYTONOTE implements our patented Lensless Technology in a tiny device design to fit within any incubator. The small foot print (132 cm² only!) allows you to follow any of your cell culture without any incubator adaptation or working space limitation.
LABEL FREE & HIGH CONTRAST
SETTINGS FREE
HUGE FIELD OF VIEW
ALWAYS IN FOCUS
CELL PROLIFERATION
CELL PROLIFERATION THROUGH CELL COUNT AND QUANTITATIVE CONFLUENCE DETERMINATION
ANGIOGENESIS
THE VERY WIDE AREA ALLOWS TO OBSERVE THE FULL ANGIOGENESIS PROCESS WITH HIGH LEVEL OF DETAILS
CELL MIGRATION
CHEMOTAXIS, WOUND HEALING ON HIGH STATISTICAL NUMBER OF CELLS AND VERY WIDE AREA
TIME-LAPSE IMAGES OF CELLS AND REAL TIME ANALYSIS FROM INSIDE YOUR INCUBATOR
The CYTONOTE 1W uses our patented lens-less imaging technology in a tiny device to fit with any incubator. The CYTONOTE 1W is the simplest live cell-imaging system designed for recording cell movies and analyzing a variety of cell culture from the incubator. The innovative and patented “Lens-less imaging” technology pushes the boundaries of microscopy with its super wide field of view and its capability to capture and analyze precisely several thousands of cells without any focus and brightness settings. This technology simplifies the Live Cell Imaging technique and transforms the complex and expensive microscope method into a cost-effective solution.
For research use only (RUO). Not for use in diagnostic procedures.
FEATURES CYTONOTE 1W
- Tiny device
- In direct from your incubator
- Time-Lapse & Movie
- Many cell cultures application
- Robust
- High Contrast
- Always in focus
- Wide field of view
CYTONOTE 1W APPLICATIONS
- Real-Time Imaging of your cell culture
- Time-Lapses and Movies of your cells
- Confluence analysis
- Growth curves
- Migration test
- Proliferation test
- Cell morphology
- Cell population
- Cell attachment / detachment
- Cell tracking
HOW TO USE IT ?
The CYTONOTE 1W is designed to be compatible with every standard lab dishes, T-flask or slides. Select your container and place it on the CYTONOTE 1W. The acquisition time between two measurements is 20 minutes by default, but you can change this time if you wish. By performing acquisitions over a give period of time you will be able to obtain time lapses and movies of your cells.
As explained, no need to adjust focus, brightness or any other parameters, our algorithm will reconstruct cells wherever cells are located… from few micrometers to some millimeters! This characteristic makes the CYTONOTE 1W an extremely robust device not fearing temperature variation, cells descent inside Matrigel, or any other parameter which can affect traditional focus adjustment.
WHAT ARE THE RESULTS ?
The CYTONOTE 1W works on an ultra-wide field of view, where all data recovered from picture represent your cell culture.
The image analysis and the result from the CYTONOTE 1W are performed from the HORUS dedicated Software. HORUS is application oriented: it provides quantitative confluence determination, cell size, cell tracking… Full field image (30mm2) of the sample is stored and can be accessed and zoomed at any time.
HORUS Software Included
Our best software for cell counting and cell analysis !
HORUS Software is included with the acquisition of our products and is regularly updated by our development team.
THE SIMULTANEOUSLY OPTION
The HORUS Software has been designed to connect up to 6 CYTONOTE 1W simultaneously allowing the control of up to 6 independent parallel cell cultures.
TECHNICAL SPECIFICATIONS
| Cells | Eucaryotic cells: adherent monolayer, suspension cell at bottom of culture ware or in micro-slides, 3D sheroids |
| Media | Liquid or semi-liquid (collagen) |
| Culture contains | Standard plastic petric dishes, culture flasks, multiwell plates, max height 55 mm |
| Resolution | 1 micron |
| Field of view | 29.4 mm² |
| Working distance | 0 to 5 mm |
| Image rate | 1.5 image/min |
| Light source | LED |
| Sensor | CMOS 10 Mpixels |
| Weight | 1 kg |
| Dimensions | 12 * 11 * 10 cm |
| Power supply | USB |
ACCESSORIES AND CONSUMABLES
| HORUS Software |
| 21 CFR |
| Scratch Assay analysis module for HORUS Software |
| Cell Tracking analysis module for HORUS Software |
| Cell Tube Formation analysis module for HORUS Software |
| Cytonote cable – 2 meters |
| Laptop computer |
| IQ/OQ |
APPLICATIONS
Quantification of Endothelial Cellular Network Formation for In-Vitro Angiogenesis Analysis
Automated Continuous Monitoring of Cell Migration (Scratch Assay) for Senescence Study
Rapid and robust cell culture confluence control for standardization of virology assays
Adherent cell culture proliferation assay in a serum comparative study with time-lapse imaging
ARTICLES
Angiogenesis
Allier C, et al. Video lensfree microscopy of 2D and 3D cultures of cells. Proc. of SPIE 2014 Vol.8947 89471H-8)
Cell Migration
Mound A, et al. Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization. Biochim Biophys Acta. 2017 Sep;1862(9):958-971
TESTIMONIES
Lamya GHENIM
CEA
Juillet 2021
“What I like most about the CYTONOTE is the tracking of thousands of cells. With the microscope, you can only follow a few hundred cells… If I could not track so many cells, I would never have been able to see this new cell rhythm. This allows the discovery of new phenomena that cannot be seen with other microscopes, and this without labelling or preparation and in direct from the incubator.
The 6-well system is excellent for parallel culture monitoring, especially when testing with drugs! It’s very convenient!
It’s a tool that is now part of our protocols! It’s essential to have several instruments to obtain all types of information. The CYTONOTE allows to bring a lot of additional information that is not possible to get with other microscopes.”