TIME-LAPSE IMAGES OF CELLS AND REAL TIME ANALYSIS FROM INSIDE YOUR INCUBATOR

CYTONOTE 1W

A small footprint device that stays always in focus with Petri dishes,
T-flasks, slides or microfluidic chips

Cytonoye 1W cut out
Cell Proliferation icon

CELL PROLIFERATION
CELL PROLIFERATION THROUGH CELL COUNT AND QUANTITATIVE CONFLUENCE DETERMINATION

ANGIOGENESIS

ANGIOGENESIS
THE VERY WIDE AREA ALLOWS TO OBSERVE THE FULL ANGIOGENESIS PROCESS WITH HIGH LEVEL OF DETAILS

cell migration icon

CELL MIGRATION
CHEMOTAXIS, WOUND HEALING ON HIGH STATISTICAL NUMBER OF CELLS AND VERY WIDE AREA

Contrast

LABEL FREE &
HIGH CONTRAST

No Settings

SETTINGS
FREE

FOV

HUGE FIELD
OF VIEW

NoZoom

ALWAYS
IN FOCUS

CYTONOTE implements our patented Lensless Technology in a tiny device design to fit within any incubator.
The small foot print (132 cm² only!) allows you to follow any of your cell culture without any incubator adaptation or working space limitation.

PRODUCT OVERVIEW – CYTONOTE 1W

TIME-LAPSE IMAGES OF CELLS AND REAL TIME ANALYSIS FROM INSIDE YOUR INCUBATOR

The CYTONOTE 1W uses our patented lens-less imaging technology in a tiny device to fit with any incubator. The CYTONOTE 1W is the simplest live cell-imaging system designed for recording cell movies and analyzing a variety of cell culture from the incubator. The innovative and patented “Lens-less imaging” technology pushes the boundaries of microscopy with its super wide field of view and its capability to capture and analyze precisely several thousands of cells without any focus and brightness settings. This technology simplifies the Live Cell Imaging technique and transforms the complex and expensive microscope method into a cost-effective solution.

cytonote 1W LIVE CELL IMAGING INSIDE THE INCUBATOR

FEATURES CYTONOTE 1W

  • Tiny device
  • In direct from your incubator
  • Time-Lapse & Movie
  • Many cell cultures application
  • Robust
  • High Contrast
  • Always in focus
  • Wide field of view

CYTONOTE 1W APPLICATIONS

  • Real-Time Imaging of your cell culture
  • Time-Lapses and Movies of your cells
  • Confluence analysis
  • Growth curves
  • Migration test
  • Proliferation test
  • Cell morphology
  • Cell population
  • Cell attachment / detachment
  • Cell tracking

HOW TO USE IT ?

Cytonote 1W and 6W LIVE CELL IMAGING INSIDE THE INCUBATOR

The CYTONOTE 1W is designed to be compatible with every standard lab dishes, T-flask or slides. Select your container and place it on the CYTONOTE 1W. The acquisition time between two measurements is 20 minutes by default, but you can change this time if you wish. By performing acquisitions over a give period of time you will be able to obtain time lapses and movies of your cells.

As explained, no need to adjust focus, brightness or any other parameters, our algorithm will reconstruct cells wherever cells are located… from few micrometers to some millimeters! This characteristic makes the CYTONOTE 1W an extremely robust device not fearing temperature variation, cells descent inside Matrigel, or any other parameter which can affect traditional focus adjustment.

WHAT ARE THE RESULTS ?

The CYTONOTE 1W works on an ultra-wide field of view, where all data recovered from picture represent your cell culture.

The image analysis and the result from the CYTONOTE 1W are performed from the HORUS dedicated Software. HORUS is application oriented: it provides quantitative confluence determination, cell size, cell tracking… Full field image (30mm2) of the sample is stored and can be accessed and zoomed at any time.

Horus software cytonote
RESULTS LIVE CELL IMAGING WITH HORUS CYTONOTE IPRASENSE
RESULTS LIVE CELL IMAGING WITH HORUS CYTONOTE IPRASENSE 02

THE SIMULTANEOUSLY OPTION

The HORUS Software has been designed to connect up to 6 CYTONOTE 1W simultaneously allowing the control of up to 6 independent parallel cell cultures.

Cytonote-6W-in-Series

TECHNICAL SPECIFICATIONS – CYTONOTE 1W

Cells Eucaryotic cells: adherent monolayer, suspension cell at bottom of culture ware or in micro-slides, 3D sheroids
Media Liquid or semi-liquid (collagen)
Culture contains Standard plastic petric dishes, culture flasks, multiwell plates, max height 55 mm
Resolution 1 micron
Field of view 29.4 mm²
Working distance 0 to 5 mm
Image rate 1.5 image/min
Light source LED
Sensor CMOS 10 Mpixels
Weight 1 kg
Dimensions 12 * 11 * 10 cm
Power supply USB

ACCESSORIES – CYTONOTE 1W

HORUS Software
21 CFR
Scratch Assay analysis module for HORUS Software
Cell Tracking analysis module for HORUS Software
Cell Tube Formation analysis module for HORUS Software
Cytonote cable – 2 meters
Laptop computer
IQ/OQ

APPLICATIONS – CYTONOTE 1W

  • Quantification of Endothelial Cellular Network Formation for In-Vitro Angiogenesis Analysis

  • Automated Continuous Monitoring of Cell Migration (Scratch Assay) for Senescence Study

  • Rapid and robust cell culture confluence control for standardization of virology assays

  • Adherent cell culture proliferation assay in a serum comparative study with time-lapse imaging

ARTICLES – CYTONOTE 1W

Angiogenesis

Allier C, et al. Video lensfree microscopy of 2D and 3D cultures of cells. Proc. of SPIE 2014 Vol.8947 89471H-8)

Cell Migration

Mound A, et al. Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization. Biochim Biophys Acta. 2017 Sep;1862(9):958-971

TESTIMONIES- CYTONOTE 6W

CEA

Lamya GHENIM

CEA

“What I like most about the CYTONOTE is the tracking of thousands of cells. With the microscope, you can only follow a few hundred cells… If I could not track so many cells, I would never have been able to see this new cell rhythm. This allows the discovery of new phenomena that cannot be seen with other microscopes, and this without labelling or preparation and in direct from the incubator.

The 6-well system is excellent for parallel culture monitoring, especially when testing with drugs! It’s very convenient!

It’s a tool that is now part of our protocols! It’s essential to have several instruments to obtain all types of information. The CYTONOTE allows to bring a lot of additional information that is not possible to get with other microscopes.”

See also our other instruments in cell count and viability

CYTONOTE 6W LIVE CELL IMAGING
CYTONOTE SCAN LIVE CELL IMAGING