TIME-LAPSE IMAGES OF CELLS AND REAL TIME ANALYSIS FROM INSIDE YOUR INCUBATOR
CYTONOTE 6W
Specifically designed for easy parallel monitoring of 6 well plate cultures
LABEL FREE & HIGH CONTRAST
SETTINGS FREE
HUGE FIELD OF VIEW
ALWAYS IN FOCUS
CELL CONFLUENCE
DETERMINATION
CELL BASED ASSAY
PROLIFERATION, MIGRATION, TRACKING, WOUND HEALING …
THE SIMPLEST LIVE CELL-IMAGING SYSTEM DESIGNED FOR THE MONITORING UP TO 6 CELL CULTURES IN PARALLEL
The CYTONOTE 6W uses our patented lens-less imaging technology in a tiny device design to fit within any incubator. The CYTONOTE product range simplifies live cell-imaging technique and transforms the complex and expensive microscope into a cost-effective solution.
The CYTONOTE 6W is the simplest live cell-imaging system designed for recording cell movies and analyzing a variety of cell culture from inside the incubator. The innovative and patented “lens-less imaging” technology pushes the boundaries of microscopy with its super wide field of view and its capability to capture and analyze precisely several thousands of cells without any focus and brightness settings.
For research use only (RUO). Not for use in diagnostic procedures.
FEATURES CYTONOTE 6W
- In direct from your incubator
- Time-Lapses & Movies
- High contrast
- Always in focus
- Settings free
- Huge field of view
- Robust
- Many cell culture application
CYTONOTE 6W APPLICATIONS
- Real-Time Imaging of your cell culture
- Up to 6 cell cultures in parallel
- Time-Lapses and Movies of your cells
- Confluence analysis
- Growth curves
- Migration test
- Proliferation test
- Cell morphology
- Cell population
- Cell attachment / detachment
- Cell tracking
HOW TO USE IT ?
The CYTONOTE 6W is designed to be compatible with every standard lab dishes, T-flask or slides. Select your container from your favorite manufacture and place it on the CYTONOTE 6W. The acquisition time between two measurements is 20 minutes by default, but you can change this time if you wish. By performing acquisitions over a given period of time you will able to obtain time-lapses and movies of your cells.
As explained, no need to adjust focus, brightness or any other parameters, our algorithm will reconstruct cells wherever cells are located… from few micrometers to some millimeters! This characteristic makes the CYTONOTE 6W an extremely robust device not fearing temperature variation, cells descent inside matrigel, or any other parameter which can affect traditional focus adjustment.
WHAT ARE THE RESULTS ?
The CYTONOTE 6W works on an ultra-wide field of view where all data recovered from picture represent your cell culture. The image analysis and results from the CYTONOTE 6W are performed by the HORUS dedicated Software. HORUS is application oriented: it provides quantitative confluence determination, cell size or cell tracking. Full field image (30mm2) of the samples are stored and can be accessed and zoomed at any time.
HORUS Software Included
Our best software for cell counting and cell analysis !
HORUS Software is included with the acquisition of our products and is regularly updated by our development team.
TECHNICAL SPECIFICATIONS
| Cells | Eucaryotic cells : adherent monolayer, suspension cell at bottom of culture ware or in micro-slides, 3D spheroids |
| Media | Liquid or semi-solid (collagen) |
| Culture Vessels | Standard plastic petri dish, culture flasks, multiwell plates, max height 55 mm |
| Resolution | 1 micron |
| Field Of View | 29.4 mm² |
| Working Distance | 0 to 5 mm |
| Image Rate | 1.5 image/minute |
| Light Source | LED 650 nm |
| Sensor | CMOS 10 Mpix |
| Dimensions | 12*11*10 cm |
| Weight | 3 Kg |
| Power Supply | USB |
ACCESSORIES AND CONSUMABLES
| HORUS Software |
| 21 CFR option for CYTONOTE 6W software |
| Remote control TCP/IP module for HORUS |
| Scratch Assay analysis module for HORUS Software |
| Cell Tracking analysis module for HORUS Software |
| Cell Tube Formation analysis module for HORUS Software |
| Cytonote cable - 2 meters |
| Incubator Gateway for Cytonote 6 Well |
| Laptop Computer |
| IQ/OQ for CYTONOTE 6W |
APPLICATIONS
Adherent cell culture proliferation assay in a serum comparative study with time-lapse imaging
Rapid and robust cell culture confluence control for standardization of virology assays
Quantification of Endothelial Cellular Network Formation for In-Vitro Angiogenesis Analysis
Automated Continuous Monitoring of Cell Migration (Scratch Assay) for Senescence Study
ARTICLES
Cell Proliferation
Allier C, et al. Imaging of dense cell cultures by multiwavelength lens-free video microscopy. Cytometry A. 2017 May; 91(5):433-442
TESTIMONIES
Lamya GHENIM
CEA
2021
“What I like most about the CYTONOTE is the tracking of thousands of cells. With the microscope, you can only follow a few hundred cells… If I could not track so many cells, I would never have been able to see this new cell rhythm. This allows the discovery of new phenomena that cannot be seen with other microscopes, and this without labelling or preparation and in direct from the incubator.
The 6-well system is excellent for parallel culture monitoring, especially when testing with drugs! It’s very convenient!
It’s a tool that is now part of our protocols! It’s essential to have several instruments to obtain all types of information. The CYTONOTE allows to bring a lot of additional information that is not possible to get with other microscopes.”