TIME-LAPSE IMAGES OF CELLS AND REAL TIME ANALYSIS FROM INSIDE YOUR INCUBATOR

CYTONOTE 6W

Specifically designed for easy parallel monitoring of 6 well plate cultures

Cytonote 6W cut out
Check

CELL CONFLUENCE
DETERMINATION

Check

CELL BASED ASSAY
PROLIFERATION, MIGRATION, TRACKING, WOUND HEALING …

Contrast

LABEL FREE
HIGH CONTRAST

No Settings

SETTINGS
FREE

FOV

HUGE FIELD
OF VIEW

NoZoom

ALWAYS
IN FOCUS

PRODUCT OVERVIEW – CYTONOTE 6W

THE SIMPLEST LIVE CELL-IMAGING SYSTEM DESIGNED FOR THE MONITORING UP TO 6 CELL CULTURES IN PARALLEL

The CYTONOTE 6W uses our patented lens-less imaging technology in a tiny device design to fit within any incubator. The CYTONOTE product range simplifies live cell-imaging technique and transforms the complex and expensive microscope into a cost-effective solution.

The CYTONOTE 6W is the simplest live cell-imaging system designed for recording cell movies and analyzing a variety of cell culture from inside the incubator. The innovative and patented “lens-less imaging” technology pushes the boundaries of microscopy with its super wide field of view and its capability to capture and analyze precisely several thousands of cells without any focus and brightness settings.

CYTONOTE 6W IPRASENSE

FEATURES OF THE CYTONOTE 6W

  • In direct from your incubator
  • Time-Lapses & Movies
  • High contrast
  • Always in focus
  • Settings free
  • Huge field of view
  • Robust
  • Many cell culture application

CYTONOTE 6W APPLICATIONS

  • Real-Time Imaging of your cell culture
  • Up to 6 cell cultures in parallel
  • Time-Lapses and Movies of your cells
  • Confluence analysis
  • Growth curves
  • Migration test
  • Proliferation test
  • Cell morphology
  • Cell population
  • Cell attachment / detachment
  • Cell tracking

HOW TO USE IT ? 

Cytonote 1W and 6W LIVE CELL IMAGING INSIDE THE INCUBATOR

The CYTONOTE 6W is designed to be compatible with every standard lab dishes, T-flask or slides. Select your container from your favorite manufacture and place it on the CYTONOTE 6W. The acquisition time between two measurements is 20 minutes by default, but you can change this time if you wish. By performing acquisitions over a given period of time you will able to obtain time-lapses and movies of your cells.

As explained, no need to adjust focus, brightness or any other parameters, our algorithm will reconstruct cells wherever cells are located… from few micrometers to some millimeters! This characteristic makes the CYTONOTE 6W an extremely robust device not fearing temperature variation, cells descent inside matrigel, or any other parameter which can affect traditional focus adjustment.

WHAT ARE THE RESULTS ?

The CYTONOTE 6W works on an ultra-wide field of view where all data recovered from picture represent your cell culture. The image analysis and results from the CYTONOTE 6W are performed by the HORUS dedicated Software. HORUS is application oriented: it provides quantitative confluence determination, cell size or cell tracking. Full field image (30mm2) of the samples are stored and can be accessed and zoomed at any time.

HORUS 6W LIVE CELL IMAGING
RESULTS LIVE CELL IMAGING WITH HORUS CYTONOTE IPRASENSE
RESULTS LIVE CELL IMAGING WITH HORUS CYTONOTE IPRASENSE 02

TECHNICAL SPECIFICATIONS – CYTONOTE 6W

Cells Eucaryotic cells : adherent monolayer, suspension cell at bottom of culture ware or in micro-slides, 3D spheroids
Media Liquid or semi-solid (collagen)
Culture Vessels Standard plastic petri dish, culture flasks, multiwell plates, max height 55 mm
Resolution 1 micron
Field Of View 29.4 mm²
Working Distance 0 to 5 mm
Image Rate 1.5 image/minute
Light Source LED 650 nm
Sensor CMOS 10 Mpix
Dimensions 12*11*10 cm
Weight 3 Kg
Power Supply USB

ACCESSORIES – CYTONOTE 6W

HORUS Software
21 CFR option for CYTONOTE 6W software
Remote control TCP/IP module for HORUS
Scratch Assay analysis module for HORUS Software
Cell Tracking analysis module for HORUS Software
Cell Tube Formation analysis module for HORUS Software
Cytonote cable - 2 meters
Incubator Gateway for Cytonote 6 Well
Laptop Computer
IQ/OQ for CYTONOTE 6W

APPLICATIONS – CYTONOTE 6W

  • Adherent cell culture proliferation assay in a serum comparative study with time-lapse imaging

  • Rapid and robust cell culture confluence control for standardization of virology assays

  • Quantification of Endothelial Cellular Network Formation for In-Vitro Angiogenesis Analysis

  • Automated Continuous Monitoring of Cell Migration (Scratch Assay) for Senescence Study

ARTICLES – CYTONOTE 6W

Cell Proliferation

Allier C, et al. Imaging of dense cell cultures by multiwavelength lens-free video microscopy. Cytometry A. 2017 May; 91(5):433-442

TESTIMONIES- CYTONOTE 6W

CEA

Lamya GHENIM

CEA

“What I like most about the CYTONOTE is the tracking of thousands of cells. With the microscope, you can only follow a few hundred cells… If I could not track so many cells, I would never have been able to see this new cell rhythm. This allows the discovery of new phenomena that cannot be seen with other microscopes, and this without labelling or preparation and in direct from the incubator.

The 6-well system is excellent for parallel culture monitoring, especially when testing with drugs! It’s very convenient!

It’s a tool that is now part of our protocols! It’s essential to have several instruments to obtain all types of information. The CYTONOTE allows to bring a lot of additional information that is not possible to get with other microscopes.”